![]() What do you need to know? Before making your dilution, make a list of things you need to know, and check it with your instructors.Make a dilution which reduces the concentration by 37-fold, which is a 1/37 dilution. You are provided with a concentrated stock solution of a yellow solute (p-nitrophenol), labeled 'NP Stock'. It is important to know how to design and perform dilutions which are accurate and which meet the needs of the situation. Reagent dilutions are needed daily in biochemical or immunological lab work. Verify instrument performance with simple tests.Download the sims 3 pc tasik game minecraft. Pros and cons of conventional spectrophotometer vs.ĩ6-well plate absorbance reader.Determine protein concentration with spectrophotometer.Determine precision and accuracy of dilutions.Understand how to calculate optimal dilutions.Spectrophotometry & Dilutions Microbiology 542 - Eric Martz Goals: 1 ml added to 9 ml gives a 10-fold dilution 1 ml added to 99ml. ![]() ![]() Serial dilution involves repeatedly mixing known amounts of source culture with (sterilised) liquid. ![]()
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